Methods for Transfecting Cells with Nucleic Acids of Animal Viruses - Dubes G. R.
Methods for Transfecting Cells with Nucleic Acids of Animal Viruses - Dubes G. R.
- A Review
AutorzyDubes G. R.
EAN: 9783034857741
Marka
Symbol
761HIS03527KS
Rok wydania
2014
Strony
84
Oprawa
Miekka
Format
17.0x24.4cm
Język
niemiecki

Bez ryzyka
14 dni na łatwy zwrot

Szeroki asortyment
ponad milion pozycji

Niskie ceny i rabaty
nawet do 50% każdego dnia
Niepotwierdzona zakupem
Ocena: /5
Marka
Symbol
761HIS03527KS
Kod producenta
9783034857741
Rok wydania
2014
Strony
84
Oprawa
Miekka
Format
17.0x24.4cm
Język
niemiecki
Autorzy
Dubes G. R.

Transfection, which is the infection of a cell with naked viral nucleic acid with the consequent production of complete virus, was first reported in 1956 for the ribonucleic acid of tobacco mosaic virus. Many reports
of transfection, and several reviews of the field, have appeared since then. Crucial for the demonstration of transfection is that the viral nucleic acid is not damaged in the process of obtaining it from the virions, or from
the infected tissue. To this end, procedures are designed to minimize the possibili ties of degradation of the viral nucleic acid by nucleases present in the biolo gical source. The most common method for preparing viral
nucleic acid is the phenol method in which virus or infected-tissue preparations are extracted with phenol. Much of the protein goes down into the phenol phase, whereas the viral nucleic acid stays up in the aqueous phase. The
transfection methods for animal virus nucleic acids are of three major kinds: (a) hypertonic methods; (b) insoluble facilitator methods; and (c) polycation methods. These methods have wide applicability to vertebrate celli
animal virus NA systems, but anyone method does not seem to be highly effective for all such systems with the possible exception of the method using the polycation diethylaminoethyl-dextran. The insoluble facilitator method
shows astonishing 'cell-specificity'; that is, it is a very effective method for transfecting some kinds of vertebrate cells, but nearly ineffective for some other kinds.
EAN: 9783034857741
EAN: 9783034857741
Niepotwierdzona zakupem
Ocena: /5
Zapytaj o produkt
Niepotwierdzona zakupem
Ocena: /5
Napisz swoją opinię