Capillary Electrophoresis - Engelhardt Heinz
Capillary Electrophoresis - Engelhardt Heinz
- Methods and Potentials
AutorzyEngelhardt Heinz
EAN: 9783642858567
Marka
Symbol
662HGK03527KS
Rok wydania
2012
Strony
228
Oprawa
Miekka
Format
17.0x24.4cm
Język
angielski

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14 dni na łatwy zwrot

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Marka
Symbol
662HGK03527KS
Kod producenta
9783642858567
Rok wydania
2012
Strony
228
Oprawa
Miekka
Format
17.0x24.4cm
Język
angielski
Autorzy
Engelhardt Heinz

Capillary electrophoresis (CE), also designated by the acronym HPCE for "High Per formance Capillary Electrophoresis" is a rapidly growing analytical separation method. It unites the separation technique of classical
electrophoresis on plates with the instrumental methods of chromatography with respect to direct detection of the solutes separated in the capillary and their ready identification and quantification. The initial problems of
inadequate reproducibility in quantitative analysis, due to the necessity of handling extremely small volumes, have largely been solved in the sec ond generation commercial instruments. Hence, a rapid and reliable separation
sys tem is available for ionic compounds from the smallest cation (the lithium ion) up to poly anions with molecular weights ranging in the millions (such as DNA molecules). The methods of gel electrophoresis and isoelectric
focusing can be readily extended to separation techniques carried out in a capillary. For nonionic compounds an addi tional separation method is available in the form of micellar electrokinetic chroma tography (MEKC). This
involves a true chromatographic separation process because the distribution of the analytes between the buffer and the micelles is superimposed on the electrophoretic migration, which contributes substantially to the selectivity.
EAN: 9783642858567
EAN: 9783642858567
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